CH 697 MASS SPECTROMETRY OF ORGANIC COMPOUNDS
Instructors
D.F. Barofsky, 1041 ALS, doug.barofsky@oregonstate.edu
C.S. Maier, 1047 ALS, claudia.maier@oregonstate.edu
Course Structure: Three credit-hour course; MWF 9AM
Office Hours: Regular hours TBA. Additional times by appointment.
Course Web Page: We will use “Blackboard” for making announcements and distributing electronic documents, etc. We recommend that you check back regularly to stay up-to-date.
Course Objective: Introduce participants to the physical principles of mass spectrometric instrumentation, MS-based analytical approaches and interpretation of mass spectra of organic compounds and biomolecules.
Course Description
A. Physical principles of mass spectrometric instrumentation (D. Barofsky)
I. Introduction
history, scope, anatomy of the mass spectrometer
II. Basic Mass Spectrometric Instrumentation
ionization modes, ion optics, mass analyzers, mass chromatography, detection and data processing
III. Basic Mass Spectrometric Methods
a. Qualitative – mass calibration (accuracy), mass resolution (precision), sensitivity, tandem mass spectrometry, library and data base searching
b. Quantitative – calibration, selected ion monitoring, selected reaction monitoring, mass chromatography
Midterm
B. Interpretation of mass spectra of organic compounds and biomolecules (C. Maier)
I. Basic Analytical Concepts
a. Isotope Abundance
b. Mass and Electron Parity
II. Electron Ionization And Fragmentation Reactions
III. Tandem Mass Spectrometric Analysis And Selected Applications
IV. Protein and Peptide Mass Spectrometry
a. Protein Mass Spectrometry
b. Mapping and Sequencing of Peptides (Proteomics)
Final Examination
Reading and Supplementary Material: Handouts, scientific articles
PREREQ: CH336, CH442