Department of Chemistry

Oregon State University

 

posted 11/29/03

Schedule for CH 663 Experiment 3 

Time

W- 11/26

M – 12/1

W- 12/3

 

ABS

FL

ABS

FL

ABS

FL

1:00

lecture

 

 

 

 

G2

2:00

G1

G4

G2

 

 

 

2:30

 

 

G3

 

 

 

3:00

 

 

G4

 

 

G3

4:00

 

 

 

 

 

G1

 

Data to be used for experiment 3, part C, part 2, this is the table specified in part a of the data analysis for calibration data  

Calibration and Precision Data Acquired on an HP Diode Array Spectrophotometer (model 8452) for Quinine Sulfate Solutions 

Concentration
(mg/mL)

Mean Absorbance
(A.U.)

Mean Transmittance

Standard Deviation of Absorbance
(A. U.)

Relative Standard Deviation in Absorbance

0.1

0.0080

0.98

0.00029

0.036

1

0.0746

0.842

0.00024

0.0032

3

0.2266

0.5933

0.00023

0.0010

10

0.7431

0.1807

0.00023

0.00031

20

1.460

0.03467

0.0010

0.00068

30

2.137

0.007295

0.0045

0.021

50

2.94

0.00115

0.025

0.0085

Unknown
1/50 dilution

0.0192

0.957

0.00025

0.0130

 

The blank standard deviation for the above data was 0.00016 A.U. from 15 measurements.  You should use these calibration data and own your blank noise data for detection limit calculations and compare results.  The calibration slope should be the same as the cell path length is the same.

You should your mean absorbance for 50 mg/mL QS to calculate the stray light fraction and compare this stray light fraction to the stray light fraction for the older instrument based on the 50 mg/mL measurement in the table above.  

Data to be used for experiment 3, part D, part 4, this is the table specified in part a of the data analysis for calibration data 

Calibration and Precision Data Acquired on an Aminco-Bowman Spectrofluorometer (Series II)) for Quinine Sulfate Solutions

Concentration
(mg/mL)

PMT Bias Voltage

Mean Total Signal

Mean Blank Signal

Analyte Fluorescence Signal

Normalized Fluorescence Signal

0.001

840

4.33

1.722

2.61

2.61

0.01

840

29.25

1.553

27.7

27.7

0.01

500

0.18

0.052

0.13

27.7

0.1

500

1.39

0.052

1.34

2.85 x 102

1

500

13.42

0.052

13.37

2.84 x 103

3

500

39.15

0.052

39.09

8.33 x 103

3

400

2.14

0.044

2.09

8.33 x 103

10

400

6.25

0.044

6.21

2.47 x 104

30

400

15.84

0.044

15.80

6.29 x 104

500

400

0.44

0.044

0.40

1.58 x 103

Unknown
1/500 dilution

400

1.58

0.044

1.54

327

 GR (500/400) = 18.7

 GR (840/500) = 213

You are using your blank standard deviations to calculate the detection limit and answer the questions about noise.  The excitation lamp was replaced since the above data were taken.  Your measurement of 1 mg/mL should be compared to that in the table and used to normalize the slope for the calculation of the detection limit.

 

 

 

posted 11/6/03, revised on 11/7/03

Revised Schedule for CH 663 Experiment 2 

Date

AAS (~1 hr)

AES (~2 hr)

W   11/5

G4

G3

F   11/7

 

G1 – 3 pm

M   11/10

G2 - 3 pm
G3 - 4 pm

W   11/12

G1 – 3 pm

G2 - 12:30 pm
G4 – 3 pm

  

Group 1

Elliot
Daniela
Judy

can meet Friday after 2 pm

Group 2

Tony
Steven
Toby

can meet Wed. from 12:30 to 3

Group 3

Carl
Watcharee
Gretchen

can meet Monday afternoon

Group 4

Myra
Yolanda
Sam

can meet  Monday afternoon

 

experiment 1B noise data on 10/29/03

one report per group of two due Monday November 3

 

Here is the PMT gain data for station 5 (the station on which we took the noise data) -  you need to use a spreadsheet to obtain the equation for the plot of log m vs log bias voltage (question 2 for the report) so you can figure out m at 850 V. This is all you need for question 1 for the report.

PMT Bias Voltage gain (m)
350 41.4
400 118
450 313

 

a - Ear

b -Edt

c - Et

c- Et

0.0171 , 1.50 s

0.0171 , 2.60 s

0.0171 , 3.64 s

0.0146 , 4.69 s

0.0171 , 5.73 s

0.0146 , 6.77 s

0.0146 , 7.82 s

0.0171 , 8.86 s

0.0171 , 9.90 s

0.0171 , 10.95 s

0.0171 , 11.99 s

0.0146 , 13.03 s

0.0171 , 14.08 s

0.0171 , 15.12 s

0.0146 , 16.17 s

0.0146 , 17.21 s

0.0171 , 18.25 s

0.0146 , 19.30 s

0.0146 , 20.34 s

0.0146 , 21.38 s

0.0171 , 22.43 s

0.0146 , 23.47 s

0.0146 , 24.51 s

0.0171 , 25.56 s

0.0171 , 26.60 s

0.0195 , 27.64 s

0.0171 , 28.69 s

0.0195 , 29.73 s

0.0195 , 30.78 s

0.0195 , 31.82 s

0.0171 , 32.86 s

0.0171 , 33.91 s

0.0171 , 34.95 s

AVG = 0.017

STD = 0.002

over 33 pts

.0391 , 1.43 s

0.0391 , 2.47 s

0.0391 , 3.52 s

0.0391 , 4.56 s

0.0366 , 5.60 s

0.0391 , 6.65 s

0.0391 , 7.69 s

0.0391 , 8.73 s

0.0415 , 9.78 s

0.0391 , 10.82 s

0.0391 , 11.87 s

0.0391 , 12.91 s

0.0391 , 13.95 s

0.0415 , 15.00 s

0.0366 , 16.04 s

0.0366 , 17.08 s

0.0366 , 18.13 s

0.0391 , 19.23 s

0.0391 , 20.27 s

0.0391 , 21.31 s

0.0366 , 22.36 s

0.0366 , 23.40 s

0.0342 , 24.44 s

0.0366 , 25.49 s

0.0366 , 26.53 s

0.0366 , 27.57 s

0.0366 , 28.62 s

0.0366 , 29.66 s

0.0366 , 30.70 s

0.0342 , 31.75 s

0.0366 , 32.79 s

0.0342 , 33.84 s

0.0366 , 34.88 s

AVG = 0.038

STD = 0.002

over 33 pts

4.7046 , 1.00 s

4.6899 , 2.04 s

4.7021 , 3.08 s

4.7095 , 4.13 s

4.7070 , 5.17 s

4.7144 , 6.21 s

4.7070 , 7.26 s

4.7168 , 8.30 s

4.6802 , 9.34 s

4.6973 , 10.39 s

4.7095 , 11.43 s

4.6826 , 12.48 s

4.7021 , 13.52 s

4.7144 , 14.56 s

4.6973 , 15.61 s

4.7095 , 16.65 s

4.6875 , 17.69 s

4.6924 , 18.74 s

4.7095 , 19.78 s

4.7070 , 20.82 s

4.7266 , 21.87 s

4.7363 , 22.91 s

4.7119 , 23.95 s

4.7021 , 25.00 s

4.7266 , 26.04 s

4.7144 , 27.09 s

4.6875 , 28.13 s

4.7021 , 29.17 s

4.7217 , 30.22 s

4.6826 , 31.31 s

4.6948 , 32.36 s

4.7314 , 33.40 s

4.7021 , 34.45 s

AVG = 4.705

STD = 0.014

over 33 pts

4.7021 , 0.80 s

4.6924 , 1.84 s

4.6899 , 2.89 s

4.6973 , 3.93 s

4.7021 , 4.97 s

4.6826 , 6.02 s

4.6924 , 7.06 s

4.6973 , 8.10 s

4.6777 , 9.15 s

4.6875 , 10.19 s

4.7119 , 11.23 s

4.6973 , 12.28 s

4.6924 , 13.32 s

4.6753 , 14.37 s

4.6899 , 15.46 s

4.6924 , 16.51 s

4.6826 , 17.55 s

4.6851 , 18.59 s

4.6802 , 19.64 s

4.7095 , 20.68 s

4.6777 , 21.73 s

4.6997 , 22.77 s

4.6948 , 23.81 s

4.6851 , 24.86 s

4.7046 , 25.90 s

4.6851 , 26.94 s

4.6973 , 27.99 s

4.6655 , 29.03 s

4.7021 , 30.07 s

4.6924 , 31.12 s

4.6851 , 32.16 s

4.6851 , 33.20 s

AVG = 4.691

STD = 0.010

over 32 pts

 

 

Below this point for fall 2002

 

 

experiment 1B noise data on 10/30/02

Here is the PMT gain data for station 6 (the station on which we took the noise data) -  you need to use a spreadsheet to obtain the equation for the plot of log m vs log bias voltage so you can figure out m at 850 V.

PMT Bias Voltage gain (m)
350 167
400 503
450 1300

 

noise data, all units are volts

a - Ear

b -Edt

c - Et

c- Et

 

 

 

 

 

 

4.3262 , 1.21 s

 

0.0049 , 0.98 s

0.0146 , 0.68 s

4.3262 , 2.25 s

4.3286 , 1.06 s

0.0024 , 2.02 s

0.0146 , 1.72 s

4.3384 , 3.29 s

4.3359 , 2.10 s

0.0024 , 3.07 s

0.0171 , 2.77 s

4.3408 , 4.34 s

4.3237 , 3.15 s

0.0049 , 4.11 s

0.0171 , 3.81 s

4.3262 , 5.38 s

4.3359 , 4.19 s

0.0024 , 5.15 s

0.0146 , 4.85 s

4.3237 , 6.43 s

4.3335 , 5.23 s

0.0049 , 6.20 s

0.0098 , 5.90 s

4.3262 , 7.47 s

4.3237 , 6.28 s

0.0049 , 7.24 s

0.0146 , 7.00 s

4.3237 , 8.51 s

4.3286 , 7.32 s

0.0049 , 8.28 s

0.0146 , 8.04 s

4.3188 , 9.56 s

4.3359 , 8.36 s

0.0000 , 9.33 s

0.0171 , 9.08 s

4.3164 , 10.60 s

4.3335 , 9.41 s

0.0024 , 10.43 s

0.0195 , 10.13 s

4.3140 , 11.64 s

4.3408 , 10.45 s

0.0000 , 11.47 s

0.0171 , 11.17 s

4.3237 , 12.69 s

4.3213 , 11.50 s

0.0049 , 12.51 s

0.0171 , 12.21 s

4.3408 , 13.73 s

4.3335 , 12.54 s

0.0049 , 13.56 s

0.0171 , 13.26 s

4.3237 , 14.83 s

4.3335 , 13.58 s

0.0024 , 14.60 s

0.0146 , 14.30 s

4.3213 , 15.87 s

4.3286 , 14.63 s

0.0049 , 15.64 s

0.0171 , 15.34 s

4.3335 , 16.92 s

4.3164 , 15.67 s

0.0000 , 16.69 s

0.0171 , 16.39 s

4.3359 , 17.96 s

4.3311 , 16.71 s

0.0049 , 17.73 s

0.0171 , 17.43 s

4.3457 , 19.00 s

4.3237 , 17.76 s

0.0098 , 18.78 s

0.0220 , 18.47 s

4.3311 , 20.05 s

4.3359 , 18.80 s

0.0024 , 19.82 s

0.0171 , 19.52 s

4.3311 , 21.09 s

4.3384 , 19.84 s

0.0024 , 20.86 s

0.0195 , 20.56 s

4.3335 , 22.13 s

4.3237 , 20.89 s

0.0024 , 21.91 s

0.0195 , 21.61 s

4.3335 , 23.18 s

4.3140 , 21.93 s

0.0000 , 22.95 s

0.0195 , 22.65 s

4.3311 , 24.22 s

4.3311 , 22.98 s

0.0024 , 23.99 s

0.0171 , 23.69 s

4.3384 , 25.27 s

4.3359 , 24.02 s

0.0073 , 25.04 s

0.0220 , 24.74 s

4.3359 , 26.31 s

4.3359 , 25.06 s

0.0122 , 26.08 s

0.0171 , 25.78 s

4.3262 , 27.35 s

4.3262 , 26.11 s

0.0024 , 27.12 s

0.0195 , 26.82 s

4.3384 , 28.40 s

4.3262 , 27.15 s

0.0000 , 28.17 s

0.0146 , 27.87 s

4.3335 , 29.44 s

4.3213 , 28.19 s

0.0000 , 29.21 s

0.0171 , 28.91 s

4.3359 , 30.48 s

4.3286 , 29.24 s

0.0024 , 30.26 s

0.0171 , 29.95 s

 

4.3335 , 30.34 s

0.0024 , 31.30 s

 

29 pt

 

 

29 pt

AVG = 4.330

29 pt

30 pt

AVG = 0.017

STD = 0.008

AVG = 4.330

AVG = 0.003

STD = 0.002

 

STD = 0.007

STD = 0.003