Determination of Vitamin B12 in Sports Drinks via HPLC. Aurea c. Chiaia, Andy J. Larkin, Tuan M. Nguyen.  2005.

Abstract:

The concentration of Vitamin B₁₂ in sports drinks was analyzed via High Performance Liquid Chromatography (HPLC) AND Diode Array was used in order to determine the absorbance of vitamin B₁₂, which was determined to be 360nm and 550nm. Solid Phase Extraction (SPE) was used to isolate vitamin B₁₂ from the sports drink matrix. A calibration curve was constructed using four standards with concentrations of 0, 20.40, and 50 ppb. The slope and the intercept were determined to be 12.6x10³ ±1.3x10³ and -23.9x10³ ±46.2x10³, respectively.